Journal: PLoS ONE
Article Title: First Description of Natural and Experimental Conjugation between Mycobacteria Mediated by a Linear Plasmid
doi: 10.1371/journal.pone.0029884
Figure Lengend Snippet: M. avium and M. kansasii colonies. (A) PFGE with undigested DNA; (B) Southern blot hybridization with IS 1245 -derived probe. Open arrow indicates pMA100; closed arrow indicates the uncharacterized smaller hybridization band. 88.1 to 88.4 = M . avium ; 88.5 to 88.75 = PCR−IS 1245- negative M . kansasii ; 88.8 to 88.15 = PCR−IS 1245- positive M. kansasii. On the left, Lambda Ladder PFG Marker (NewEngland BioLabs) molecular size markers.
Article Snippet: PFGE was carried out in a CHEF-DR III system (Bio-Rad) at 14°C for 24 h at 6 V.cm −1 with switch times of 1.6 to 21.3 s. Bacteriophage lambda ladder PFG marker (New England BioLabs, Ipswitch, MA) was used as molecular standard.
Techniques: Southern Blot, Hybridization, Derivative Assay, Marker